Microbiology: Laboratory Theory & Application, 4e

By Michael J. Leboffe, Burton E. Pierce  •  
•  912
Format • 
Print ISBN  9781617312502
• eBook ISBN  9781617314186
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Beautiful full-color photography, effective illustrations, carefully written exercises, and a reasonable price combine to make Microbiology: Laboratory Theory & Application the best-selling microbiology lab manual series on the market. This edition satisfies the content needs of courses populated by majors or in nonmajors courses where an increased coverage of applied microbiology is desired.

The following grid illustrates the primary audience and coverage differences between the three titles in the Microbiology: Laboratory Theory and Application series.

Examples of interior pages:


What's New in this Edition
Table of Contents
Instructor Resources
  • Contains 119 exercises covering a full selection of clinical and applied microbiology topics not found in the briefer versions of the manual, including:
    • Microscopic Examination of Freshwater Microbes
    • Reduction Potential
    • Clinical Biofilms
    • Winogradsky Column
    • Nitrogen Fixation
    • Soil Slide Culture
    • Restriction Digest
    • Polymerase Chain Reaction
    • Phage Typing of E. coli Strains
    • ELISA for Detecting Antibodies in a Patient's Sample
  • Theory and Application provide students with the general principles and everyday purpose of the labs.
  • In This Exercise clearly explains what students can expect as they work through the Lab Procedures.
  • Biochemical reactions for the organism and indicator reactions of the test depict the reactions taking place in the lab.
  • MaterialsMedium Recipes, and Procedures give students step-by-step instructions for conducting the labs. Biosafety levels are also included so that students are aware of the safety precautions necessary for each lab.
  • Tables of results help students organize and interpret results.
  • Data sheets provide students room to record their data and answer critical thinking questions.
  • High-qualityfull-color photographsmicrographs, and illustrations ensure that students have color-accurate visual representations of what they will see in the lab.
  • Photographs and procedural diagrams outline the steps necessary to correctly complete the lab.
  • Photographs with directional arrows help ensure students use proper technique.
  • Clearly written, informative captions help ensure students use proper technique in the lab.

Instructor Resources Available: Login to view Instructor Resources

  • An online Instructor's Manual contains tips for the instructor and answers to all of the exercises.
  • All of the images from the lab manual are provided electronically. Images are presented at digital display resolution (150 dpi), intended for use in PowerPoint presentations.
  • Digital Test Bank contains questions to help instructors build tests and quizzes that can either be printed or exported to commonly used distance learning platforms.
What's New in this Edition
  • Increased emphasis on good technique to minimize aerosol production
  • Reduction in the number of BSL-2 organisms used, and where possible, replaced with suitable substitutes that provide the same result
  • Data sheets have been moved from the back of the book to immediately follow the corresponding exercise
  • New and revised photographs and artwork throughout
  • Many exercises have been revised, re-organized, or enhanced in order to improve clarity, reflect current taxonomy, and reflect CDC changes
  • New content includes:
    • Sample “safety contract” for students
    • Section devoted exclusively to handing BSL-2 organisms
    • Hand washing exercise
    • T-streak inoculation
    • Instruction on Köhler illumination for the light microscope
    • Exercise on Yogurt CFU Density of an Active Live Culture and Identification of a Yogurt Unknown
    • Heavily revised exercises on Restriction Digest and Polymerase Chain Reaction now utilize “from scratch” protocols replacing commercial kit
Table of contents
Introduction: Safety and Laboratory Guidelines
Section 1: Fundamental Skills for the Microbiology Laboratory
Exercise 1-1: A Comparison of Hand-Cleansing Agents
Exercise 1-2: Nutrient Broth and Nutrient Agar Preparation
Exercise 1-3: Common Aseptic Transfers and Inoculation Methods (includes pg. 27)
Exercise 1-4: Streak Plate Methods of Isolation
Exercise 1-5: Spread Plate Method of Isolation
Section 1 Introductory Pages
Section 2: Microbial Growth
Exercise 2-1: Ubiquity of Microorganisms
Exercise 2-2: Colony Morphology
Exercise 2-3: Growth Patterns on Slants
Exercise 2-4: Growth Patterns in Broth
Exercise 2-5: Evaluation of Media
Exercise 2-6: Agar Deep Stabs
Exercise 2-7: Fluid Thioglycollate Broth
Exercise 2-8: Anaerobic Jar
Exercise 2-9: The Effect of Temperature on Microbial Growth
Exercise 2-10: The Effect of pH on Microbial Growth
Exercise 2-11: The Effect of Osmotic Pressure on Microbial Growth
Exercise 2-12: Steam Sterilization (includes pg. 121)
Exercise 2-13: The Lethal Effect of Ultraviolet Radiation on Microbial Growth
Exercise 2-14: Chemical Germicides: Disinfectants and Antiseptics
Section 2 Introductory Pages
Section 3: Microscopy and Staining
Exercise 3-1: Introduction to the Light Microscope (includes pg. 142)
Exercise 3-2: Calibration of the Ocular Micrometer
Exercise 3-3: Microscopic Examination of Eukaryotic Microbes
Exercise 3-4: Microscopic Examination of Freshwater Microbes
Exercise 3-5: Simple Stains (includes pgs. 181-184)
Exercise 3-6: Negative Stains
Exercise 3-7: Gram Stain
Exercise 3-8: Acid-Fast Stains
Exercise 3-9: Capsule Stain
Exercise 3-10: Endospore Stain
Exercise 3-11: Wet Mount and Hanging Drop Preparations
Exercise 3-12: Flagella Stain
Exercise 3-13: Morphological Unknown
Section 3 Introductory Pages
Section 4: Selective Media
Exercise 4-1: Phenylethyl Alcohol Agar
Exercise 4-2: Columbia CNA With 5% Sheep Blood Agar
Exercise 4-3: Bile Esculin Agar
Exercise 4-4: Mannitol Salt Agar
Exercise 4-5: MacConkey Agar
Exercise 4-6: Eosin Methylene Blue Agar (Levine)
Exercise 4-7: Hektoen Enteric Agar
Exercise 4-8: Xylose Lysine Desoxycholate Agar
Section 4 Introductory Pages
Section 5: Differential Tests
Exercise 5-1: Reduction Potential
Exercise 5-2: Oxidation-Fermentation Test
Exercise 5-3: Phenol Red Broth
Exercise 5-4: Methyl Red and Voges-Proskauer Tests
Exercise 5-5: ONPG Test
Exercise 5-6: Catalase Test
Exercise 5-7: Oxidase Test
Exercise 5-8: Nitrate Reduction Test
Exercise 5-9: Citrate Utilization Test
Exercise 5-10: Malonate Utilization Test
Exercise 5-11: Decarboxylation Tests
Exercise 5-12: Phenylalanine Deaminase test
Exercise 5-13: Starch Hydrolysis (Amylase Test)
Exercise 5-14: DNA Hydrolysis (DNase Test)
Exercise 5-15: Lipid Hydrolysis (Lipase Test)
Exercise 5-16: Casein Hydrolysis (Casease Test)
Exercise 5-17: Gelatin Hydrolysis (Gelatinase Test)
Exercise 5-18: Urea Hydrolysis (Urease Test)
Exercise 5-19: PYR Test
Exercise 5-20: SIM Medium (Sulfur Reduction, Indole Production, Motility)
Exercise 5-21: Triple Sugar Iron Agar / Kligler Iron Agar
Exercise 5-22: Lysine Iron Agar
Exercise 5-23: Litmus Milk Medium
Exercise 5-24: Bacitracin, Novobiocin, and Optochin Susceptibility Tests
Exercise 5-25: Blood Agar
Exercise 5-26: Camp Test
Exercise 5-27: Coagulase and Clumping Factor Tests
Exercise 5-28: Motility Test
Exercise 5-29: api 20 E Identification System for Enterobacteriaceae
Exercise 5-30: EnteroPluri-Test (Enterotube® II)
Exercise 5-31: Bacterial Unknowns Project
Section 5 Introductory Pages
Section 6: Quantitative Techniques
Exercise 6-1: Standard Plate Count (Viable Count)
Exercise 6-2: Urine Culture
Exercise 6-3: Direct Count (Petroff-Hausser)
Exercise 6-4: Closed-System Growth
Exercise 6-5: Plaque Assay of Virus Titre
Exercise 6-6: Thermal Death Time Versus Decimal Reduction Value
Section 6 Introductory Pages
Section 7: Medical Microbiology
Exercise 7-1: Snyder Test
Exercise 7-2: Lysozyme Assay
Exercise 7-3: Antimicrobial Susceptibility Test: Disk Diffusion (Kirby-Bauer) Method
Exercise 7-4: Clinical Biofilms
Exercise 7-5: Morbidity and Mortality Weekly Report (MMWR) Assignment
Exercise 7-6: Epidemic Simulation
Exercise 7-7: Identification of Enterobacteriaceae
Exercise 7-8: Identification of Gram-positive Cocci
Exercise 7-9: Identification of Gram-positive Rods
Section 7 Introductory Pages
Section 8: Environmental Microbiology
Exercise 8-1: Winogradsky Column
Exercise 8-2: Nitrogen Fixation (includes pgs. 589-590)
Exercise 8-3: Nitrification: The Production of Nitrate
Exercise 8-4: Ammonification
Exercise 8-5: Denitrification: Nitrate Reduction
Exercise 8-6: Photosynthetic Sulfur Bacteria (includes pgs. 613-614)
Exercise 8-7: Chemolithotrophic Sulfur-Oxidizing Bacteria
Exercise 8-8: Sulfur-Reducing Bacteria
Exercise 8-9: Bioluminescence
Exercise 8-10: Soil Slide Culture
Exercise 8-11: Soil Microbial Count
Exercise 8-12: Membrane Filter Technique
Exercise 8-13: Multiple Tube Fermentation Method for Determining Water Quality
Section 8 Introductory Pages
Section 9: Food Microbiology
Exercise 9-1: Methylene Blue Reductase Test
Exercise 9-2: Making Yogurt
Exercise 9-3: Yogurt CFU Density of an Active and Live Culture and Identification of a Yogurt Unknown
Section 9 Introductory Pages
Section 10: Microbial Genetics
Exercise 10-1: Extraction of DNA from Bacterial Cells
Exercise 10-2: Restriction Digest
Exercise 10-3: Bacterial Transformation: The pGLOTM System
Exercise 10-4: Polymerase Chain Reaction
Exercise 10-5: Ultraviolet Radiation Damage and Repair
Exercise 10-6: Ames Test
Exercise 10-7: Phage Typing of E. coli Strains
Section 10 Introductory Pages
Section 11: Hematology and Serology
Exercise 11-1: Differential Blood Cell Count
Exercise 11-2: Precipitin Ring Test (includes pgs. 751-752)
Exercise 11-3: Double Gel Immunodiffusion
Exercise 11-4: Slide Agglutination
Exercise 11-5: Blood Typing
Exercise 11-6: ELISA for Detecting Antibodies in a Patient's Sample (The Antibody Capture Method)
Section 11 Introductory Pages
Section 12: Eukaryotic Microbes
Exercise 12-1: Representative Fungi of Medical and Economic Importance
Exercise 12-2: Fungal Slide Culture
Exercise 12-3: Examination of Simple Eukaryotic Pathogens
Exercise 12-4: Parasitic Helminths
Section 12 Introductory Pages
Appendix A: Biochemical Pathways
Appendix B: Miscellaneous Transfer Methods
Appendix C: Transfer from a Broth Culture Using a Glass Pipette
Appendix D: Transfer from a Broth Culture Using a Digital Pipette
Appendix E: The Spectrophotometer
Appendix F: Alternative Procedures for Section 6
Appendix G: Agarose Gel Electrophoresis
Appendix H: Medium, Reagent, and Stain Recipes
Instructor Resources
  • An online Instructor's Manual contains tips for the instructor and answers to all of the exercises.
  • All of the images from the lab manual are provided electronically. Images are presented at digital display resolution (150 dpi), intended for use in PowerPoint presentations.
  • Digital Test Bank contains questions to help instructors build tests and quizzes that can either be printed or exported to commonly used distance learning platforms.