Exercises for the Microbiology Laboratory, 5e

By Burton E. Pierce, Michael J. Leboffe  •  
•  280
Format • 
Print ISBN  9781617319044
• eBook ISBN  9781617319075
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This inexpensive exercise manual provides a straightforward, step-by-step, concise alternative to large microbiology laboratory manuals. It can be used by itself as a required lab text and is also designed to be used in conjunction with A Photographic Atlas for the Microbiology Laboratory, 5e, by Leboffe & Pierce, with exercises keyed to specific images.

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What's New in this Edition
Table of Contents
Versions and Packages
  • Exercises are arranged by functional group within each section, allowing you to select the exercises you want to teach within a set of lab outcomes.
  • Frequent page and figure number references for the Photographic Atlas for the Microbiology Laboratory are included in each section.
  • A useful appendix containing recipes for all media, stains, and reagents used helps students and instructors with lab preparation.
  • The organization of the Exercises and the Atlas helps students learn basic steps and techniques prior to attempting and applying more complicated techniques.
What's New in this Edition
  • Provides an even greater emphasis on lab safety including a student “Safety Contract”.
  • Reduced number of BSL-2 organisms to a minimum. For labs where BSL-2 organisms will be used, a new section on their safe handling has been included.
  • Resequencing of the sections to match the order of the equivalent sections in the photographic atlas.
  • Exercises revised in order to provide more clear and complete lab instructions.
Table of contents
INTRODUCTION Safety and Laboratory Guidelines
Student Conduct
Basic Laboratory Safety
Reducing Contamination of Self, Others, Cultures, and the Environment
Guidelines Governing Handling of BSL-2 Organisms
Disposing of Contaminated Materials
Student Compliance with Laboratory Safety Regulations
Student Safety Contract
SECTION 1 Fundamental Skills for the Microbiology Laboratory
Exercise 1-1 Nutrient Broth and Nutrient Agar Preparation
Exercise 1-2 Common Aseptic Transfers and Inoculation Methods
SECTION 2 Isolation Techniques and Selective Media
Exercise 2-1 Streak Plate Methods of Isolation
Exercise 2-2 Spread Plate Method of Isolation
Exercise 2-3 Mannitol Salt Agar (MSA)
Exercise 2-4 Phenylethyl Alcohol (PEA) Agar
Exercise 2-5 Desoxycholate (DOC) Agar
Exercise 2-6 mEndo Agar LES
Exercise 2-7 Eosin Methylene Blue (EMB) Agar (Levine)
Exercise 2-8 MacConkey Aga
Exercise 2-9 Hektoen Enteric (HE) Agar
Exercise 2-10 Xylose Lysine Desoxycholate (XLD) Agar
SECTION 3 Microbial Growth
Exercise 3-1 Ubiquity of Microorganisms
Exercise 3-2 Colony Morphology
Exercise 3-3 Growth Patterns on Agar Slants
Exercise 3-4 Growth Patterns in Broths
Exercise 3-5 Evaluation of Media
Exercise 3-6 Agar Deep Stab
Exercise 3-7 Agar Shakes
Exercise 3-8 Anaerobic Jar
Exercise 3-9 Fluid Thioglycollate Broth
Exercise 3-10 The Effect of Temperature on Microbial Growth
Exercise 3-11 The Effect of pH on Microbial Growth
Exercise 3-12 The Effect of Osmotic Pressure on Microbial Growth
Exercise 3-13 The Effect of Ultraviolet Radiation on Microbial Growth
Exercise 3-14 Effectiveness of Chemical Germicides: The Use-Dilution Test for Disinfectants and Antiseptics
SECTION 4 Microscopy and Staining
Exercise 4-1 Introduction to the Light Microscope
Exercise 4-2 Calibration of the Ocular Micrometer
Exercise 4-3 Microscopic Examination of Eukaryotic Microbes
Exercise 4-4 Simple Stains
Exercise 4-5 Negative Stains
Exercise 4-6 Gram Stain
Exercise 4-7 Acid-Fast Stains
Exercise 4-8 Capsule Stain
Exercise 4-9 Endospore Stain
Exercise 4-10 Bacterial Motility: Wet Mount and Hanging Drop Preparations
Exercise 4-11 Morphological Unknown
SECTION 5: Differential Tests
Exercise 5-1 Oxidation–Fermentation (O–F) Test
Exercise 5-2 Phenol Red Fermentation (PR) Broth
Exercise 5-3 Purple Broth
Exercise 5-4 Methyl Red (MR) and Voges-Proskauer (VP) Tests
Exercise 5-5 Catalase Test
Exercise 5-6 Oxidase Test
Exercise 5-7 Nitrate Reduction Test
Exercise 5-8 Citrate Utilization Test
Exercise 5-9 Malonate Utilization Test
Exercise 5-10 Amino Acid Decarboxylation (Decarboxylase Tests)
Exercise 5-11 Phenylalanine Deaminase Test
Exercise 5-12 Bile Esculin Agar (Esculinase Test)
Exercise 5-13 Starch Hydrolysis (Amylase Test)
Exercise 5-14 Urea Hydrolysis (Urease Test)
Exercise 5-15 Casein Hydrolysis (Casease Test)
Exercise 5-16 Gelatin Hydrolysis (Gelatinase Test)
Exercise 5-17 DNA Hydrolysis (DNase Test)
Exercise 5-18 Lipid Hydrolysis (Lipase Test)
Exercise 5-19 SIM Medium: Determination of Sulfur Reduction, Indole Production, and Motility
Exercise 5-20 Triple Sugar Iron Agar (TSIA)/Kligler Iron Agar (KIA)
Exercise 5-21 Lysine Iron Agar (LIA)
Exercise 5-22 Litmus Milk Medium
Exercise 5-23 Bacitracin Susceptibility Tests
Exercise 5-24 β-Lactamase Test
Exercise 5-25 Blood Agar
Exercise 5-26 Coagulase Tube Test and Clumping Factor Test
Exercise 5-27 Motility Agar
Exercise 5-28 Bacterial Unknowns Project
SECTION 6 Hematology and Serology
Exercise 6-1 Differential Blood Cell Count
Exercise 6-2 Precipitin Ring Test
Exercise 6-3 Double Gel Immunodiffusion
Exercise 6-4 Slide Agglutination: The Salmonella O Antigen
Exercise 6-5 Blood Typing
SECTION 7 Microbial Genetics
Exercise 7-1 Extraction of DNA from Escherichia coli Cells
Exercise 7-2 Ultraviolet Radiation Damage and Repair
Exercise 7-3 Ames Test
SECTION 8 Eukaryotic Microbes: Fungi, Protozoans, and Helminths
Exercise 8-1 Fungi
Exercise 8-2 Protozoans
Exercise 8-3 Parasitic Helminths
SECTION 9 Quantitative Techniques
Exercise 9-1 Standard Plate Count (Viable Count)
Exercise 9-2 Direct Count (Petroff-Hausser Counting Chamber)
Exercise 9-3 Plaque Assay of Virus Titer
Exercise 9-4 Closed System Growth
Exercise 9-5 Urine Streak–Semiquantitative Method
SECTION 10 Medical, Environmental, and Food Microbiology
Exercise 10-1 Antimicrobial Susceptibility Test: Disk Diffusion (Kirby-Bauer) Method
Exercise 10-2 Snyder Test
Exercise 10-3 Lysozyme Assay
Exercise 10-4 Membrane Filter Technique
Exercise 10-5 Multiple Tube Fermentation Method for Total Coliform Determination
Exercise 10-6 Bioluminescence
Exercise 10-7 Soil Microbial Count
Exercise 10-8 Methylene Blue Reductase Test (MBRT)
Exercise 10-9 Viable Cell Preservatives
Exercise 10-10 Making Yogurt
Appendix A: Medium, Reagent, and Stain Recipes
Appendix B: Miscellaneous Transfer Methods
Appendix C: Transfers Using a Glass Pipette
Appendix D: Transfer from a Broth Culture Using a Digital Pipette
Appendix E: The Spectrophotometer