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Microbiology: Laboratory Theory & Application, 4e

By Michael J. Leboffe • © 01/01/2015 • 912 Pages
Loose-Leaf Format • Print ISBN 9781617312502 • eBook ISBN9781617314186

Table of contents

Introduction: Safety and Laboratory Guidelines
14 Pages
Section 1: Fundamental Skills for the Microbiology Laboratory
Exercise 1-1: A Comparison of Hand-Cleansing Agents
4 Pages
Exercise 1-2: Nutrient Broth and Nutrient Agar Preparation
6 Pages
Exercise 1-3: Common Aseptic Transfers and Inoculation Methods (includes pg. 27)
14 Pages
Exercise 1-4: Streak Plate Methods of Isolation
8 Pages
Exercise 1-5: Spread Plate Method of Isolation
6 Pages
Section 1 Introductory Pages
2 Pages
Section 2: Microbial Growth
Exercise 2-1: Ubiquity of Microorganisms
6 Pages
Exercise 2-2: Colony Morphology
12 Pages
Exercise 2-3: Growth Patterns on Slants
4 Pages
Exercise 2-4: Growth Patterns in Broth
4 Pages
Exercise 2-5: Evaluation of Media
8 Pages
Exercise 2-6: Agar Deep Stabs
4 Pages
Exercise 2-7: Fluid Thioglycollate Broth
4 Pages
Exercise 2-8: Anaerobic Jar
4 Pages
Exercise 2-9: The Effect of Temperature on Microbial Growth
6 Pages
Exercise 2-10: The Effect of pH on Microbial Growth
4 Pages
Exercise 2-11: The Effect of Osmotic Pressure on Microbial Growth
8 Pages
Exercise 2-12: Steam Sterilization (includes pg. 121)
8 Pages
Exercise 2-13: The Lethal Effect of Ultraviolet Radiation on Microbial Growth
6 Pages
Exercise 2-14: Chemical Germicides: Disinfectants and Antiseptics
6 Pages
Section 2 Introductory Pages
2 Pages
Section 3: Microscopy and Staining
Exercise 3-1: Introduction to the Light Microscope (includes pg. 142)
13 Pages
Exercise 3-2: Calibration of the Ocular Micrometer
6 Pages
Exercise 3-3: Microscopic Examination of Eukaryotic Microbes
8 Pages
Exercise 3-4: Microscopic Examination of Freshwater Microbes
12 Pages
Exercise 3-5: Simple Stains (includes pgs. 181-184)
10 Pages
Exercise 3-6: Negative Stains
4 Pages
Exercise 3-7: Gram Stain
8 Pages
Exercise 3-8: Acid-Fast Stains
8 Pages
Exercise 3-9: Capsule Stain
4 Pages
Exercise 3-10: Endospore Stain
6 Pages
Exercise 3-11: Wet Mount and Hanging Drop Preparations
4 Pages
Exercise 3-12: Flagella Stain
4 Pages
Exercise 3-13: Morphological Unknown
6 Pages
Section 3 Introductory Pages
1 Pages
Section 4: Selective Media
Exercise 4-1: Phenylethyl Alcohol Agar
6 Pages
Exercise 4-2: Columbia CNA With 5% Sheep Blood Agar
6 Pages
Exercise 4-3: Bile Esculin Agar
4 Pages
Exercise 4-4: Mannitol Salt Agar
6 Pages
Exercise 4-5: MacConkey Agar
8 Pages
Exercise 4-6: Eosin Methylene Blue Agar (Levine)
6 Pages
Exercise 4-7: Hektoen Enteric Agar
6 Pages
Exercise 4-8: Xylose Lysine Desoxycholate Agar
6 Pages
Section 4 Introductory Pages
3 Pages
Section 5: Differential Tests
Exercise 5-1: Reduction Potential
6 Pages
Exercise 5-2: Oxidation-Fermentation Test
8 Pages
Exercise 5-3: Phenol Red Broth
8 Pages
Exercise 5-4: Methyl Red and Voges-Proskauer Tests
6 Pages
Exercise 5-5: ONPG Test
4 Pages
Exercise 5-6: Catalase Test
6 Pages
Exercise 5-7: Oxidase Test
6 Pages
Exercise 5-8: Nitrate Reduction Test
6 Pages
Exercise 5-9: Citrate Utilization Test
6 Pages
Exercise 5-10: Malonate Utilization Test
4 Pages
Exercise 5-11: Decarboxylation Tests
8 Pages
Exercise 5-12: Phenylalanine Deaminase test
4 Pages
Exercise 5-13: Starch Hydrolysis (Amylase Test)
6 Pages
Exercise 5-14: DNA Hydrolysis (DNase Test)
4 Pages
Exercise 5-15: Lipid Hydrolysis (Lipase Test)
4 Pages
Exercise 5-16: Casein Hydrolysis (Casease Test)
4 Pages
Exercise 5-17: Gelatin Hydrolysis (Gelatinase Test)
4 Pages
Exercise 5-18: Urea Hydrolysis (Urease Test)
6 Pages
Exercise 5-19: PYR Test
4 Pages
Exercise 5-20: SIM Medium (Sulfur Reduction, Indole Production, Motility)
8 Pages
Exercise 5-21: Triple Sugar Iron Agar / Kligler Iron Agar
6 Pages
Exercise 5-22: Lysine Iron Agar
4 Pages
Exercise 5-23: Litmus Milk Medium
6 Pages
Exercise 5-24: Bacitracin, Novobiocin, and Optochin Susceptibility Tests
6 Pages
Exercise 5-25: Blood Agar
6 Pages
Exercise 5-26: Camp Test
4 Pages
Exercise 5-27: Coagulase and Clumping Factor Tests
4 Pages
Exercise 5-28: Motility Test
4 Pages
Exercise 5-29: api 20 E Identification System for Enterobacteriaceae
8 Pages
Exercise 5-30: EnteroPluri-Test (Enterotube® II)
8 Pages
Exercise 5-31: Bacterial Unknowns Project
10 Pages
Section 5 Introductory Pages
4 Pages
Section 6: Quantitative Techniques
Exercise 6-1: Standard Plate Count (Viable Count)
10 Pages
Exercise 6-2: Urine Culture
4 Pages
Exercise 6-3: Direct Count (Petroff-Hausser)
4 Pages
Exercise 6-4: Closed-System Growth
10 Pages
Exercise 6-5: Plaque Assay of Virus Titre
10 Pages
Exercise 6-6: Thermal Death Time Versus Decimal Reduction Value
10 Pages
Section 6 Introductory Pages
2 Pages
Section 7: Medical Microbiology
Exercise 7-1: Snyder Test
4 Pages
Exercise 7-2: Lysozyme Assay
6 Pages
Exercise 7-3: Antimicrobial Susceptibility Test: Disk Diffusion (Kirby-Bauer) Method
8 Pages
Exercise 7-4: Clinical Biofilms
4 Pages
Exercise 7-5: Morbidity and Mortality Weekly Report (MMWR) Assignment
10 Pages
Exercise 7-6: Epidemic Simulation
6 Pages
Exercise 7-7: Identification of Enterobacteriaceae
8 Pages
Exercise 7-8: Identification of Gram-positive Cocci
8 Pages
Exercise 7-9: Identification of Gram-positive Rods
8 Pages
Section 7 Introductory Pages
2 Pages
Section 8: Environmental Microbiology
Exercise 8-1: Winogradsky Column
6 Pages
Exercise 8-2: Nitrogen Fixation (includes pgs. 589-590)
10 Pages
Exercise 8-3: Nitrification: The Production of Nitrate
6 Pages
Exercise 8-4: Ammonification
4 Pages
Exercise 8-5: Denitrification: Nitrate Reduction
4 Pages
Exercise 8-6: Photosynthetic Sulfur Bacteria (includes pgs. 613-614)
8 Pages
Exercise 8-7: Chemolithotrophic Sulfur-Oxidizing Bacteria
6 Pages
Exercise 8-8: Sulfur-Reducing Bacteria
6 Pages
Exercise 8-9: Bioluminescence
4 Pages
Exercise 8-10: Soil Slide Culture
4 Pages
Exercise 8-11: Soil Microbial Count
6 Pages
Exercise 8-12: Membrane Filter Technique
8 Pages
Exercise 8-13: Multiple Tube Fermentation Method for Determining Water Quality
10 Pages
Section 8 Introductory Pages
2 Pages
Section 9: Food Microbiology
Exercise 9-1: Methylene Blue Reductase Test
4 Pages
Exercise 9-2: Making Yogurt
4 Pages
Exercise 9-3: Yogurt CFU Density of an Active and Live Culture and Identification of a Yogurt Unknown
10 Pages
Section 9 Introductory Pages
2 Pages
Section 10: Microbial Genetics
Exercise 10-1: Extraction of DNA from Bacterial Cells
6 Pages
Exercise 10-2: Restriction Digest
12 Pages
Exercise 10-3: Bacterial Transformation: The pGLOTM System
8 Pages
Exercise 10-4: Polymerase Chain Reaction
14 Pages
Exercise 10-5: Ultraviolet Radiation Damage and Repair
6 Pages
Exercise 10-6: Ames Test
6 Pages
Exercise 10-7: Phage Typing of E. coli Strains
4 Pages
Section 10 Introductory Pages
2 Pages
Section 11: Hematology and Serology
Exercise 11-1: Differential Blood Cell Count
6 Pages
Exercise 11-2: Precipitin Ring Test (includes pgs. 751-752)
6 Pages
Exercise 11-3: Double Gel Immunodiffusion
4 Pages
Exercise 11-4: Slide Agglutination
4 Pages
Exercise 11-5: Blood Typing
6 Pages
Exercise 11-6: ELISA for Detecting Antibodies in a Patient's Sample (The Antibody Capture Method)
10 Pages
Section 11 Introductory Pages
2 Pages
Section 12: Eukaryotic Microbes
Exercise 12-1: Representative Fungi of Medical and Economic Importance
12 Pages
Exercise 12-2: Fungal Slide Culture
6 Pages
Exercise 12-3: Examination of Simple Eukaryotic Pathogens
12 Pages
Exercise 12-4: Parasitic Helminths
14 Pages
Section 12 Introductory Pages
2 Pages
Appendix A: Biochemical Pathways
8 Pages
Appendix B: Miscellaneous Transfer Methods
4 Pages
Appendix C: Transfer from a Broth Culture Using a Glass Pipette
4 Pages
Appendix D: Transfer from a Broth Culture Using a Digital Pipette
4 Pages
Appendix E: The Spectrophotometer
4 Pages
Appendix F: Alternative Procedures for Section 6
6 Pages
Appendix G: Agarose Gel Electrophoresis
6 Pages
Appendix H: Medium, Reagent, and Stain Recipes
18 Pages
Glossary
8 Pages
Index
8 Pages

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