You are here

Microbiology: Laboratory Theory & Application, Brief, 3e

Michael J. Leboffe, Burton E. Pierce
Suggested Student Price: 
Customize this Book Request Review Copy
Product Code: 
Product Format: 

This manual is appropriate for courses populated primarily by allied health students or for courses where an abbreviated number of experiments is preferred. This new edition has been carefully revised to provide increased clarity, better organization, and improvements to its already unsurpassed photography and artwork. These features have made this manual and its associated title, Microbiology: Laboratory Theory & Application, 4e, the best-selling microbiology lab manual series on the market. 

About this Product


  • Theory and Application provide students with the general principles and everyday purpose of the labs. 
  • In This Exercise clearly explains what students can expect as they work through the Lab Procedures. 
  • Biochemical Reactions for the Organism and Indicator Reactions of the Test depict the reactions taking place in the lab. 
  • Materials, Medium Recipes, and Lab Procedures give students step-by-step instructions for conducting the labs. Biosafety levels are also included so that students are aware of the safety precautions necessary for each lab. 
  • Tables of Results help students organize and interpret results. 
  • Data Sheets provide students room to record their data and answer critical thinking questions.
  • High-quality, full-color photographs, micrographs, and illustrations ensure that students have color-accurate visual representations of what they will see in the lab. 
  • Photographs and Procedural Diagrams outline the steps necessary to correctly complete the lab. 
  • Photographs with directional arrows help ensure students use proper technique. 
  • Clearly written, informative captions help ensure students use proper technique in the lab.

About the Author

Michael J. Leboffe

Burton E. Pierce

What's New in this Edition

New to this edition of Microbiology: Laboratory Theory & Application, Brief, 3e

  • Exercises are appropriate for both majors and nonmajors courses and have been selected to provide exposure to lab experiences from all areas of microbiology. 
  • This version features more than 300 full-color, high-quality, color-accurate photographs, micrographs, and illustrations. 
  • Where possible, biosafety level (BSL) 2 organisms have been replaced with equivalent BSL-1 organisms. 
  • Data sheets are incorporated into the experiments for ease of use. 
  • The lab manual emphasizes the content and inclusion of skills as advised by the American Society for Microbiology in their Laboratory Core Curriculum. 

Table of Contents


Introduction: Safety and Laboratory Guidelines

Section 1: Fundamental Skills for the Microbiology Laboratory

Section 2: Microbial Growth

Section 3: Microscopy and Staining

Section 4: Selective Media

Section 5: Differential Tests

Section 6: Quantitative Techniques

Section 7: Medical, Environmental, and Food Microbiology

Section 8: Microbial Genetics and Serology

Section 9: Identification of Unknowns

Appendix A: Biochemical Pathways

Appendix B: Miscellaneous Transfer Methods

Appendix C: Transfers Using a Glass Pipette

Appendix D: Transfers from a Broth Culture Using a Digital Pipette

Appendix E: Medium, Reagent, and Stain Recipes