Table of Contents # of Pages Select Introduction: Safety and Laboratory Guidelines 14

Section 1: Fundamental Skills for the Microbiology Laboratory 44

Section 1: Introductory Pages 2

Exercise 1-1: Glo Germ™ Hand Wash Education System 4

Exercise 1-2: A Comparison of Hand-Cleansing Agents 4

Exercise 1-3: Nutrient Broth and Nutrient Agar Preparation 6

Exercise 1-4: Common Aseptic Transfers and Inoculation Methods 14

Exercise 1-5: Streak Plate Methods of Isolation 8

Exercise 1-6: Spread Plate Method of Isolation 6

Section 2: Microbial Growth 82

Section 2: Introductory Pages 2

Exercise 2-1: Ubiquity of Microorganisms 6

Exercise 2-2: Colony Morphology 12

Exercise 2-3: Growth Patterns on Slants 4

Exercise 2-4: Growth Patterns in Broth 4

Exercise 2-5: Evaluation of Media 8

Exercise 2-6: Fluid Thioglycollate Medium 6

Exercise 2-7: Anaerobic Jar 4

Exercise 2-8: The Effect of Temperature on Microbial Growth 6

Exercise 2-9: The Effect of pH on Microbial Growth 6

Exercise 2-10: The Effect of Osmotic Pressure on Microbial Growth 6

Physical and Chemical Methods of Pathogen Control 2

Exercise 2-11: Steam Sterilization 6

Exercise 2-12: The Effect of Ultraviolet Radiation on Microbial Growth 4

Exercise 2-13: Effectiveness of Chemical Germicides: The Use-Dilation Test for Disinfectants and Antiseptics 6

Section 3: Microscopy and Staining 86

Section 3: Introductory Pages 1

Exercise 3-1: Introduction to the Light Microscope (includes p. 142) 11

Exercise 3-2: Calibration of the Ocular Micrometer 6

Exercise 3-3: Microscopic Examination of Eukaryotic Microbes 14

Exercise 3-4: Simple Stains (includes pg. 173-176) 10

Exercise 3-5: Negative Stains 4

Exercise 3-6: Gram Stain 8

Exercise 3-7: Acid-Fast Stains 6

Exercise 3-8: Capsule Stain 4

Exercise 3-9: Endospore Stain 6

Exercise 3-10: Bacterial Motility: Wet Mount and Hanging Drop Preparations 6

Exercise 3-11: Bacterial Motility: Flagella 4

Exercise 3-12: Morphological Unknown 6

Section 4: Selective Media 40

Section 4: Introductory Pages 2

Exercise 4-1: Phenylethyl Alcohol Agar 6

Exercise 4-2: Columbia CNA with 5% Sheep Blood Agar 6

Exercise 4-3: Mannitol Salt Agar 6

Exercise 4-4: MacConkey Agar 8

Exercise 4-5: Eosin Methylene Blue Agar 6

Exercise 4-6: Hektoen Enteric Agar 6

Section 5: Differential Tests 138

Section 5: Introductory Pages 4

Exercise 5-1: Oxidation-Fermentation (O-F) Test 8

Exercise 5-2: Phenol Red Fermentation Broth 8

Exercise 5-3: Methyl Red and Voges-Proskauer Tests 8

Exercise 5-4: Catalase Test 6

Exercise 5-5: Oxidase Test 6

Exercise 5-6: Nitrate Reduction Test 6

Exercise 5-7: Citrate Utilization Test 6

Exercise 5-8: Amino Acid Decarboxylation (Decarboxylase Tests) 8

Exercise 5-9: Phenylalanine Deaminase Test 4

Exercise 5-10: Starch Hydrolysis (Amylase Test) 6

Exercise 5-11: DNA Hydrolysis (DNase Test) 4

Exercise 5-12: Lipid Hydrolysis (Lipase Test) 4

Exercise 5-13: Casein Hydrolysis (Casease Test) 4

Exercise 5-14: Gelatin Hydrolysis (Gelatinase Test) 4

Exercise 5-15: Urea Hydrolysis (Urease Test) 4

Exercise 5-16: Bile Esculin Test 4

Exercise 5-17: L-pyrrolidonyl-B-naphthylamide Hydrolysis (PYR Test) 4

Exercise 5-18: SIM Medium: Determination of Sulfur Reduction, Indole Production, and Motility 8

Exercise 5-19: Triple Sugar Iron Agar/Kligler Iron Agar 6

Exercise 5-20: Bacitracin, Novobiocin and Optochin Susceptibility Tests 6

Exercise 5-21: Blood Agar 6

Exercise 5-22: CAMP Test 4

Exercise 5-23: Coagulase and Clumping Factor Tests 6

Exercise 5-24: Motility Agar 4

Section 6: Quantitative Techniques 36

Section 6: Introductory Pages 2

Exercise 6-1: Environmental Sampling: The RODAC™ Plate 4

Exercise 6-2: Standard Plate Count (Viable Count) 10

Exercise 6-3: Urine Culture 4

Exercise 6-4: Plaque Assay of Virus Titer 10

Exercise 6-5: Differential Blood Cell Count 6

Section 7: Medical, Environmental, and Food Microbiology 58

Section 7: Introductory Pages 2

Exercise 7-1: Snyder Test 4

Exercise 7-2: Antimicrobial Susceptibility Test: Disk Diffusion (Kirby-Bauer) Method 8

Exercise 7-3: Morbidity and Mortality Weekly Report (MMWR) Assignment 10

Exercise 7-4: Epidemic Simulation 8

Exercise 7-5: Membrane Filter Technique 8

Exercise 7-6: Multiple Tube Fermentation Method for Total Coliform Determination 10

Exercise 7-7: Methylene Blue Reductase Test (MBRT) 4

Exercise 7-8: Making Yogurt 4

Section 8: Microbial Genetics and Serology 46

Section 8: Introductory Pages 2

Exercise 8-1: Extraction of DNA from Escherichia coli Cells 6

Exercise 8-2: Bacterial Transformation: the pGLO™ System 8

Exercise 8-3: Ultraviolet Radiation Damage and Repair 6

Introduction to Antigens and Antibodies & Serological Reactions 2

Exercise 8-4: Slide Agglutination: The Salmonella O Antigen 6

Exercise 8-5: Blood Typing 6

Exercise 8-6: ELISA For Detecting Antibodies in a Patient's Sample (The Antibody Capture Method) 10

Section 9: Identification of Unknowns 44

Section 9: Introductory Pages 2

Exercise 9-1: Identification of Selected Enterobacteriaceae 10

Exercise 9-2: Identification of Selected Gram-Positive Cocci 8

Exercise 9-3: Identification of Selected Gram-Positive Rods 8

Exercise 9-4: api® 20 E Identification System for Enterobacteriaceae and Other Gram-Negative Rods 8

Exercise 9-5: EnteroPluri-Test 8

Appendix A: Biochemical Pathways 10

Appendix B: Miscellaneous Transfer Methods 4

Appendix C: Transfers Using a Glass Pipette 4

Appendix D: Transfers from a Broth Culture Using a Digital Pipette 4

Appendix E: Medium, Reagent, and Stain Recipes 12

Glossary 10

Index 10