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Microbiology: Laboratory Theory & Application, Brief, 3e

Michael J. Leboffe, Burton E. Pierce
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Table of Contents# of PagesSelect
Introduction: Safety and Laboratory Guidelines14
Section 1: Fundamental Skills for the Microbiology Laboratory44
Section 1: Introductory Pages2
Exercise 1-1: Glo Germ™ Hand Wash Education System4
Exercise 1-2: A Comparison of Hand-Cleansing Agents4
Exercise 1-3: Nutrient Broth and Nutrient Agar Preparation6
Exercise 1-4: Common Aseptic Transfers and Inoculation Methods14
Exercise 1-5: Streak Plate Methods of Isolation8
Exercise 1-6: Spread Plate Method of Isolation6
Section 2: Microbial Growth82
Section 2: Introductory Pages2
Exercise 2-1: Ubiquity of Microorganisms6
Exercise 2-2: Colony Morphology12
Exercise 2-3: Growth Patterns on Slants4
Exercise 2-4: Growth Patterns in Broth4
Exercise 2-5: Evaluation of Media8
Exercise 2-6: Fluid Thioglycollate Medium6
Exercise 2-7: Anaerobic Jar4
Exercise 2-8: The Effect of Temperature on Microbial Growth6
Exercise 2-9: The Effect of pH on Microbial Growth6
Exercise 2-10: The Effect of Osmotic Pressure on Microbial Growth6
Physical and Chemical Methods of Pathogen Control2
Exercise 2-11: Steam Sterilization6
Exercise 2-12: The Effect of Ultraviolet Radiation on Microbial Growth4
Exercise 2-13: Effectiveness of Chemical Germicides: The Use-Dilation Test for Disinfectants and Antiseptics6
Section 3: Microscopy and Staining86
Section 3: Introductory Pages1
Exercise 3-1: Introduction to the Light Microscope (includes p. 142)11
Exercise 3-2: Calibration of the Ocular Micrometer6
Exercise 3-3: Microscopic Examination of Eukaryotic Microbes14
Exercise 3-4: Simple Stains (includes pg. 173-176)10
Exercise 3-5: Negative Stains4
Exercise 3-6: Gram Stain8
Exercise 3-7: Acid-Fast Stains6
Exercise 3-8: Capsule Stain4
Exercise 3-9: Endospore Stain6
Exercise 3-10: Bacterial Motility: Wet Mount and Hanging Drop Preparations6
Exercise 3-11: Bacterial Motility: Flagella4
Exercise 3-12: Morphological Unknown6
Section 4: Selective Media40
Section 4: Introductory Pages2
Exercise 4-1: Phenylethyl Alcohol Agar6
Exercise 4-2: Columbia CNA with 5% Sheep Blood Agar6
Exercise 4-3: Mannitol Salt Agar6
Exercise 4-4: MacConkey Agar8
Exercise 4-5: Eosin Methylene Blue Agar6
Exercise 4-6: Hektoen Enteric Agar6
Section 5: Differential Tests138
Section 5: Introductory Pages4
Exercise 5-1: Oxidation-Fermentation (O-F) Test8
Exercise 5-2: Phenol Red Fermentation Broth8
Exercise 5-3: Methyl Red and Voges-Proskauer Tests8
Exercise 5-4: Catalase Test6
Exercise 5-5: Oxidase Test6
Exercise 5-6: Nitrate Reduction Test6
Exercise 5-7: Citrate Utilization Test6
Exercise 5-8: Amino Acid Decarboxylation (Decarboxylase Tests)8
Exercise 5-9: Phenylalanine Deaminase Test4
Exercise 5-10: Starch Hydrolysis (Amylase Test)6
Exercise 5-11: DNA Hydrolysis (DNase Test)4
Exercise 5-12: Lipid Hydrolysis (Lipase Test)4
Exercise 5-13: Casein Hydrolysis (Casease Test)4
Exercise 5-14: Gelatin Hydrolysis (Gelatinase Test)4
Exercise 5-15: Urea Hydrolysis (Urease Test)4
Exercise 5-16: Bile Esculin Test4
Exercise 5-17: L-pyrrolidonyl-B-naphthylamide Hydrolysis (PYR Test)4
Exercise 5-18: SIM Medium: Determination of Sulfur Reduction, Indole Production, and Motility8
Exercise 5-19: Triple Sugar Iron Agar/Kligler Iron Agar6
Exercise 5-20: Bacitracin, Novobiocin and Optochin Susceptibility Tests6
Exercise 5-21: Blood Agar6
Exercise 5-22: CAMP Test4
Exercise 5-23: Coagulase and Clumping Factor Tests6
Exercise 5-24: Motility Agar4
Section 6: Quantitative Techniques36
Section 6: Introductory Pages2
Exercise 6-1: Environmental Sampling: The RODAC™ Plate4
Exercise 6-2: Standard Plate Count (Viable Count)10
Exercise 6-3: Urine Culture4
Exercise 6-4: Plaque Assay of Virus Titer10
Exercise 6-5: Differential Blood Cell Count6
Section 7: Medical, Environmental, and Food Microbiology58
Section 7: Introductory Pages2
Exercise 7-1: Snyder Test4
Exercise 7-2: Antimicrobial Susceptibility Test: Disk Diffusion (Kirby-Bauer) Method8
Exercise 7-3: Morbidity and Mortality Weekly Report (MMWR) Assignment10
Exercise 7-4: Epidemic Simulation8
Exercise 7-5: Membrane Filter Technique8
Exercise 7-6: Multiple Tube Fermentation Method for Total Coliform Determination10
Exercise 7-7: Methylene Blue Reductase Test (MBRT)4
Exercise 7-8: Making Yogurt4
Section 8: Microbial Genetics and Serology46
Section 8: Introductory Pages2
Exercise 8-1: Extraction of DNA from Escherichia coli Cells6
Exercise 8-2: Bacterial Transformation: the pGLO™ System8
Exercise 8-3: Ultraviolet Radiation Damage and Repair6
Introduction to Antigens and Antibodies & Serological Reactions2
Exercise 8-4: Slide Agglutination: The Salmonella O Antigen6
Exercise 8-5: Blood Typing6
Exercise 8-6: ELISA For Detecting Antibodies in a Patient's Sample (The Antibody Capture Method)10
Section 9: Identification of Unknowns44
Section 9: Introductory Pages2
Exercise 9-1: Identification of Selected Enterobacteriaceae10
Exercise 9-2: Identification of Selected Gram-Positive Cocci8
Exercise 9-3: Identification of Selected Gram-Positive Rods8
Exercise 9-4: api® 20 E Identification System for Enterobacteriaceae and Other Gram-Negative Rods8
Exercise 9-5: EnteroPluri-Test8
Appendix A: Biochemical Pathways10
Appendix B: Miscellaneous Transfer Methods4
Appendix C: Transfers Using a Glass Pipette4
Appendix D: Transfers from a Broth Culture Using a Digital Pipette4
Appendix E: Medium, Reagent, and Stain Recipes12