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Microbiology: Laboratory Theory & Application, 4e

Michael J. Leboffe, Burton E. Pierce
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Table of Contents# of PagesSelect
Introduction: Safety and Laboratory Guidelines14
Section 1: Fundamental Skills for the Microbiology Laboratory40
Section 1 Introductory Pages2
Exercise 1-1: A Comparison of Hand-Cleansing Agents4
Exercise 1-2: Nutrient Broth and Nutrient Agar Preparation6
Exercise 1-3: Common Aseptic Transfers and Inoculation Methods (includes pg. 27)14
Exercise 1-4: Streak Plate Methods of Isolation8
Exercise 1-5: Spread Plate Method of Isolation6
Section 2: Microbial Growth86
Section 2 Introductory Pages2
Exercise 2-1: Ubiquity of Microorganisms6
Exercise 2-2: Colony Morphology12
Exercise 2-3: Growth Patterns on Slants4
Exercise 2-4: Growth Patterns in Broth4
Exercise 2-5: Evaluation of Media8
Exercise 2-6: Agar Deep Stabs4
Exercise 2-7: Fluid Thioglycollate Broth4
Exercise 2-8: Anaerobic Jar4
Exercise 2-9: The Effect of Temperature on Microbial Growth6
Exercise 2-10: The Effect of pH on Microbial Growth4
Exercise 2-11: The Effect of Osmotic Pressure on Microbial Growth8
Exercise 2-12: Steam Sterilization (includes pg. 121)8
Exercise 2-13: The Lethal Effect of Ultraviolet Radiation on Microbial Growth6
Exercise 2-14: Chemical Germicides: Disinfectants and Antiseptics6
Section 3: Microscopy and Staining94
Section 3 Introductory Pages1
Exercise 3-1: Introduction to the Light Microscope (includes pg. 142)13
Exercise 3-2: Calibration of the Ocular Micrometer6
Exercise 3-3: Microscopic Examination of Eukaryotic Microbes8
Exercise 3-4: Microscopic Examination of Freshwater Microbes12
Exercise 3-5: Simple Stains (includes pgs. 181-184)10
Exercise 3-6: Negative Stains4
Exercise 3-7: Gram Stain8
Exercise 3-8: Acid-Fast Stains8
Exercise 3-9: Capsule Stain4
Exercise 3-10: Endospore Stain6
Exercise 3-11: Wet Mount and Hanging Drop Preparations4
Exercise 3-12: Flagella Stain4
Exercise 3-13: Morphological Unknown6
Section 4: Selective Media50
Section 4 Introductory Pages3
Exercise 4-1: Phenylethyl Alcohol Agar6
Exercise 4-2: Columbia CNA With 5% Sheep Blood Agar6
Exercise 4-3: Bile Esculin Agar4
Exercise 4-4: Mannitol Salt Agar6
Exercise 4-5: MacConkey Agar8
Exercise 4-6: Eosin Methylene Blue Agar (Levine)6
Exercise 4-7: Hektoen Enteric Agar6
Exercise 4-8: Xylose Lysine Desoxycholate Agar6
Section 5: Differential Tests182
Section 5 Introductory Pages4
Exercise 5-1: Reduction Potential6
Exercise 5-2: Oxidation-Fermentation Test8
Exercise 5-3: Phenol Red Broth8
Exercise 5-4: Methyl Red and Voges-Proskauer Tests6
Exercise 5-5: ONPG Test4
Exercise 5-6: Catalase Test6
Exercise 5-7: Oxidase Test6
Exercise 5-8: Nitrate Reduction Test6
Exercise 5-9: Citrate Utilization Test6
Exercise 5-10: Malonate Utilization Test4
Exercise 5-11: Decarboxylation Tests8
Exercise 5-12: Phenylalanine Deaminase test4
Exercise 5-13: Starch Hydrolysis (Amylase Test)6
Exercise 5-14: DNA Hydrolysis (DNase Test)4
Exercise 5-15: Lipid Hydrolysis (Lipase Test)4
Exercise 5-16: Casein Hydrolysis (Casease Test)4
Exercise 5-17: Gelatin Hydrolysis (Gelatinase Test)4
Exercise 5-18: Urea Hydrolysis (Urease Test)6
Exercise 5-19: PYR Test4
Exercise 5-20: SIM Medium (Sulfur Reduction, Indole Production, Motility)8
Exercise 5-21: Triple Sugar Iron Agar / Kligler Iron Agar6
Exercise 5-22: Lysine Iron Agar4
Exercise 5-23: Litmus Milk Medium6
Exercise 5-24: Bacitracin, Novobiocin, and Optochin Susceptibility Tests6
Exercise 5-25: Blood Agar6
Exercise 5-26: Camp Test4
Exercise 5-27: Coagulase and Clumping Factor Tests4
Exercise 5-28: Motility Test4
Exercise 5-29: api 20 E Identification System for Enterobacteriaceae8
Exercise 5-30: EnteroPluri-Test (Enterotube® II)8
Exercise 5-31: Bacterial Unknowns Project10
Section 6: Quantitative Techniques50
Section 6 Introductory Pages2
Exercise 6-1: Standard Plate Count (Viable Count)10
Exercise 6-2: Urine Culture4
Exercise 6-3: Direct Count (Petroff-Hausser)4
Exercise 6-4: Closed-System Growth10
Exercise 6-5: Plaque Assay of Virus Titre10
Exercise 6-6: Thermal Death Time Versus Decimal Reduction Value10
Section 7: Medical Microbiology64
Section 7 Introductory Pages2
Exercise 7-1: Snyder Test4
Exercise 7-2: Lysozyme Assay6
Exercise 7-3: Antimicrobial Susceptibility Test: Disk Diffusion (Kirby-Bauer) Method8
Exercise 7-4: Clinical Biofilms4
Exercise 7-5: Morbidity and Mortality Weekly Report (MMWR) Assignment10
Exercise 7-6: Epidemic Simulation6
Exercise 7-7: Identification of Enterobacteriaceae8
Exercise 7-8: Identification of Gram-positive Cocci8
Exercise 7-9: Identification of Gram-positive Rods8
Section 8: Environmental Microbiology84
Section 8 Introductory Pages2
Exercise 8-1: Winogradsky Column6
Exercise 8-2: Nitrogen Fixation (includes pgs. 589-590)10
Exercise 8-3: Nitrification: The Production of Nitrate6
Exercise 8-4: Ammonification4
Exercise 8-5: Denitrification: Nitrate Reduction4
Exercise 8-6: Photosynthetic Sulfur Bacteria (includes pgs. 613-614)8
Exercise 8-7: Chemolithotrophic Sulfur-Oxidizing Bacteria6
Exercise 8-8: Sulfur-Reducing Bacteria6
Exercise 8-9: Bioluminescence4
Exercise 8-10: Soil Slide Culture4
Exercise 8-11: Soil Microbial Count6
Exercise 8-12: Membrane Filter Technique8
Exercise 8-13: Multiple Tube Fermentation Method for Determining Water Quality10
Section 9: Food Microbiology20
Section 9 Introductory Pages2
Exercise 9-1: Methylene Blue Reductase Test4
Exercise 9-2: Making Yogurt4
Exercise 9-3: Yogurt CFU Density of an Active and Live Culture and Identification of a Yogurt Unknown10
Section 10: Microbial Genetics58
Section 10 Introductory Pages2
Exercise 10-1: Extraction of DNA from Bacterial Cells6
Exercise 10-2: Restriction Digest12
Exercise 10-3: Bacterial Transformation: The pGLOTM System8
Exercise 10-4: Polymerase Chain Reaction14
Exercise 10-5: Ultraviolet Radiation Damage and Repair6
Exercise 10-6: Ames Test6
Exercise 10-7: Phage Typing of E. coli Strains4
Section 11: Hematology and Serology38
Section 11 Introductory Pages2
Exercise 11-1: Differential Blood Cell Count6
Exercise 11-2: Precipitin Ring Test (includes pgs. 751-752)6
Exercise 11-3: Double Gel Immunodiffusion4
Exercise 11-4: Slide Agglutination4
Exercise 11-5: Blood Typing6
Exercise 11-6: ELISA for Detecting Antibodies in a Patient's Sample (The Antibody Capture Method)10
Section 12: Eukaryotic Microbes46
Section 12 Introductory Pages2
Exercise 12-1: Representative Fungi of Medical and Economic Importance12
Exercise 12-2: Fungal Slide Culture6
Exercise 12-3: Examination of Simple Eukaryotic Pathogens12
Exercise 12-4: Parasitic Helminths14
Appendix A: Biochemical Pathways8
Appendix B: Miscellaneous Transfer Methods4
Appendix C: Transfer from a Broth Culture Using a Glass Pipette4
Appendix D: Transfer from a Broth Culture Using a Digital Pipette4
Appendix E: The Spectrophotometer4
Appendix F: Alternative Procedures for Section 66
Appendix G: Agarose Gel Electrophoresis6
Appendix H: Medium, Reagent, and Stain Recipes18