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Microbiology: Laboratory Theory & Application, Brief, 3e

By Michael J. Leboffe • © 01/01/2016 • 656 Pages
Loose-leaf Format • Print ISBN 9781617314773 • eBook ISBN9781617315602

Table of contents

Introduction: Safety and Laboratory Guidelines
14 Pages
Section 1: Fundamental Skills for the Microbiology Laboratory
Section 1: Introductory Pages
2 Pages
Exercise 1-1: Glo Germ™ Hand Wash Education System
4 Pages
Exercise 1-2: A Comparison of Hand-Cleansing Agents
4 Pages
Exercise 1-3: Nutrient Broth and Nutrient Agar Preparation
6 Pages
Exercise 1-4: Common Aseptic Transfers and Inoculation Methods
14 Pages
Exercise 1-5: Streak Plate Methods of Isolation
8 Pages
Exercise 1-6: Spread Plate Method of Isolation
6 Pages
Section 2: Microbial Growth
Section 2: Introductory Pages
2 Pages
Exercise 2-1: Ubiquity of Microorganisms
6 Pages
Exercise 2-2: Colony Morphology
12 Pages
Exercise 2-3: Growth Patterns on Slants
4 Pages
Exercise 2-4: Growth Patterns in Broth
4 Pages
Exercise 2-5: Evaluation of Media
8 Pages
Exercise 2-6: Fluid Thioglycollate Medium
6 Pages
Exercise 2-7: Anaerobic Jar
4 Pages
Exercise 2-8: The Effect of Temperature on Microbial Growth
6 Pages
Exercise 2-9: The Effect of pH on Microbial Growth
6 Pages
Exercise 2-10: The Effect of Osmotic Pressure on Microbial Growth
6 Pages
Physical and Chemical Methods of Pathogen Control
2 Pages
Exercise 2-11: Steam Sterilization
6 Pages
Exercise 2-12: The Effect of Ultraviolet Radiation on Microbial Growth
4 Pages
Exercise 2-13: Effectiveness of Chemical Germicides: The Use-Dilation Test for Disinfectants and Antiseptics
6 Pages
Section 3: Microscopy and Staining
Section 3: Introductory Pages
1 Pages
Exercise 3-1: Introduction to the Light Microscope (includes p. 142)
11 Pages
Exercise 3-2: Calibration of the Ocular Micrometer
6 Pages
Exercise 3-3: Microscopic Examination of Eukaryotic Microbes
14 Pages
Exercise 3-4: Simple Stains (includes pg. 173-176)
10 Pages
Exercise 3-5: Negative Stains
4 Pages
Exercise 3-6: Gram Stain
8 Pages
Exercise 3-7: Acid-Fast Stains
6 Pages
Exercise 3-8: Capsule Stain
4 Pages
Exercise 3-9: Endospore Stain
6 Pages
Exercise 3-10: Bacterial Motility: Wet Mount and Hanging Drop Preparations
6 Pages
Exercise 3-11: Bacterial Motility: Flagella
4 Pages
Exercise 3-12: Morphological Unknown
6 Pages
Section 4: Selective Media
Section 4: Introductory Pages
2 Pages
Exercise 4-1: Phenylethyl Alcohol Agar
6 Pages
Exercise 4-2: Columbia CNA with 5% Sheep Blood Agar
6 Pages
Exercise 4-3: Mannitol Salt Agar
6 Pages
Exercise 4-4: MacConkey Agar
8 Pages
Exercise 4-5: Eosin Methylene Blue Agar
6 Pages
Exercise 4-6: Hektoen Enteric Agar
6 Pages
Section 5: Differential Tests
Section 5: Introductory Pages
4 Pages
Exercise 5-1: Oxidation-Fermentation (O-F) Test
8 Pages
Exercise 5-2: Phenol Red Fermentation Broth
8 Pages
Exercise 5-3: Methyl Red and Voges-Proskauer Tests
8 Pages
Exercise 5-4: Catalase Test
6 Pages
Exercise 5-5: Oxidase Test
6 Pages
Exercise 5-6: Nitrate Reduction Test
6 Pages
Exercise 5-7: Citrate Utilization Test
6 Pages
Exercise 5-8: Amino Acid Decarboxylation (Decarboxylase Tests)
8 Pages
Exercise 5-9: Phenylalanine Deaminase Test
4 Pages
Exercise 5-10: Starch Hydrolysis (Amylase Test)
6 Pages
Exercise 5-11: DNA Hydrolysis (DNase Test)
4 Pages
Exercise 5-12: Lipid Hydrolysis (Lipase Test)
4 Pages
Exercise 5-13: Casein Hydrolysis (Casease Test)
4 Pages
Exercise 5-14: Gelatin Hydrolysis (Gelatinase Test)
4 Pages
Exercise 5-15: Urea Hydrolysis (Urease Test)
4 Pages
Exercise 5-16: Bile Esculin Test
4 Pages
Exercise 5-17: L-pyrrolidonyl-B-naphthylamide Hydrolysis (PYR Test)
4 Pages
Exercise 5-18: SIM Medium: Determination of Sulfur Reduction, Indole Production, and Motility
8 Pages
Exercise 5-19: Triple Sugar Iron Agar/Kligler Iron Agar
6 Pages
Exercise 5-20: Bacitracin, Novobiocin and Optochin Susceptibility Tests
6 Pages
Exercise 5-21: Blood Agar
6 Pages
Exercise 5-22: CAMP Test
4 Pages
Exercise 5-23: Coagulase and Clumping Factor Tests
6 Pages
Exercise 5-24: Motility Agar
4 Pages
Section 6: Quantitative Techniques
Section 6: Introductory Pages
2 Pages
Exercise 6-1: Environmental Sampling: The RODAC™ Plate
4 Pages
Exercise 6-2: Standard Plate Count (Viable Count)
10 Pages
Exercise 6-3: Urine Culture
4 Pages
Exercise 6-4: Plaque Assay of Virus Titer
10 Pages
Exercise 6-5: Differential Blood Cell Count
6 Pages
Section 7: Medical, Environmental, and Food Microbiology
Section 7: Introductory Pages
2 Pages
Exercise 7-1: Snyder Test
4 Pages
Exercise 7-2: Antimicrobial Susceptibility Test: Disk Diffusion (Kirby-Bauer) Method
8 Pages
Exercise 7-3: Morbidity and Mortality Weekly Report (MMWR) Assignment
10 Pages
Exercise 7-4: Epidemic Simulation
8 Pages
Exercise 7-5: Membrane Filter Technique
8 Pages
Exercise 7-6: Multiple Tube Fermentation Method for Total Coliform Determination
10 Pages
Exercise 7-7: Methylene Blue Reductase Test (MBRT)
4 Pages
Exercise 7-8: Making Yogurt
4 Pages
Section 8: Microbial Genetics and Serology
Section 8: Introductory Pages
2 Pages
Exercise 8-1: Extraction of DNA from Escherichia coli Cells
6 Pages
Exercise 8-2: Bacterial Transformation: the pGLO™ System
8 Pages
Exercise 8-3: Ultraviolet Radiation Damage and Repair
6 Pages
Introduction to Antigens and Antibodies & Serological Reactions
2 Pages
Exercise 8-4: Slide Agglutination: The Salmonella O Antigen
6 Pages
Exercise 8-5: Blood Typing
6 Pages
Exercise 8-6: ELISA For Detecting Antibodies in a Patient's Sample (The Antibody Capture Method)
10 Pages
Section 9: Identification of Unknowns
Section 9: Introductory Pages
2 Pages
Exercise 9-1: Identification of Selected Enterobacteriaceae
10 Pages
Exercise 9-2: Identification of Selected Gram-Positive Cocci
8 Pages
Exercise 9-3: Identification of Selected Gram-Positive Rods
8 Pages
Exercise 9-4: api® 20 E Identification System for Enterobacteriaceae and Other Gram-Negative Rods
8 Pages
Exercise 9-5: EnteroPluri-Test
8 Pages
Appendix A: Biochemical Pathways
10 Pages
Appendix B: Miscellaneous Transfer Methods
4 Pages
Appendix C: Transfers Using a Glass Pipette
4 Pages
Appendix D: Transfers from a Broth Culture Using a Digital Pipette
4 Pages
Appendix E: Medium, Reagent, and Stain Recipes
12 Pages
Glossary
10 Pages
Index
10 Pages

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